Chii chinonzi PCR Analysis?
Polymerase chain reaction (PCR) inyanzvi inzira ye laboratori yakagadzirirwa kutarisa maduku maduku eDNA mune imwe sampuro nenzira inonzi inosimudzira . Munguva yePCR kuwedzera, DNA inofarira inonyorwa kakawanda kusvikira pane zvakakwana zvekuongorora uye kuonekwa. Semuenzaniso, PCR inogona kushandiswa kujekesa maduku maduku eDNA kubva kune zvipenyu zvinokonzera gonorrhea kana chlamydia zviripo mumuenzaniso soro .
Ko PCR Inoshanda Sei?
Nhanho yekutanga muPCR ndeyokupisa shanho kuitira kuti DNA yakanyanyisa-diki iparadzaniswe muzvigaro zviviri - izvi zvinonzi denaturation . Zvadaro zvinotora , zviduku zviduku zveDNA zvinokwana kusvika kumagumo eDNA zvakarongeka, zvinosanganiswa neDNA samuenzaniso. Mushure meizvi, DNA polymerase inoshandiswa kutanga kudzokorora DNA panzvimbo yekutanga. Pakupedzisira, DNA inopisa kuti iparadzanise michina zvakare, uye iyo yose PCR inotanga zvakare.
Chiyero cheDNA chikamu chechido chiripo mune sampuko inowedzera exponentially nega yega purogiramu yePCR: kopi imwe inova mbiri, zvino inova ina, zvino inova masere, nezvimwe; saka kazhinji, zvinongoda kusvika makumi maviri kusvika makumi mana chete zvinodiwa kuti uone kana DNA iri mubvunzo iripo (uye, kana iri, kupa samu yakakwana yekuongorora).
Zvose matanho e-polymerase chain reaction - kuratidza DNA, kushandisa zvinotangira, uye kuwirirana kweDNA-inoitika pamatambo akasiyana-siyana, saka mushure mekunge musanganiswa wokutanga wakaiswa pamwe chete, matanho anokwanisa kudzorwa kuburikidza neitiro inozivikanwa se thermocycling , umo inotonhorera inobatwa pamatunhu anodiwa kwenguva yakareba zvakakwana kuti danho rimwe nerimwe riitwe.
Nokudaro, PCR inzira inobatsira yekusimudzira chiyero cheDNA mune imwe yero test tube isina zvishoma zvido zvekupindira kwevanhu.
Polymerase chain reaction yakaratidza revolution muzvigadzirwa zvezvinyorwa apo yakatanga kutanga mumakore ekuma1980, uye muumbi weCCR, Kary Mullis, akakunda Nobel Prize muKemistry nokuda kwebasa rake muna 1993.
Nei PCR Inotarisana neHIV Testing?
Polymerase chain reaction, uye michina yakabatana yakadai se ligase chain reaction , iri kuratidza kukosha kukura kwekuongorora kweSDD. Ichi ndechokuti maitiro aya anogona kunyatsojekesa maduku madzimai eDNA kana RNA muzvidzidzo. Kuziva hutachiona hwepathogen hazvidi kuti pathogen ive yakararama - semaketeteria tsika kana chirwere chetachiona . Izvi hazvirevi kuti hutachiona huve hwakaitika nguva yakareba yakapfuura kuti vanhu vave vakagadzira kuonekwa kwevanhu vanorwisa (sekuonekwa kunoonekwa neE ELISA .) Izvi zvinoreva kuti maPC techniques anogona dzimwe nguva kuona zvirwere mberi kwevamwe kuedzwa, uye pasina zvakanyanya kufanira kuve nehanya nekuchengeta samples ari mupenyu kana kuedza panguva yakakodzera.